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Svec is deleterious for both the instrument and the separation. Second, while managing the flow rate, the well-known effect of flow rate on column efficiency most often demonstrated by the van Deemter plot also has to be considered. Although the mass transfer term plays a much smaller role in CEC, the flow rate used for the separation in a specific column packed with porous beads should match that at the minimum of the curve. The situation is different for very small non-porous particles for which the A and C terms of the van Deemter equation can be neglected and only the axial diffusion effectively affects the column efficiency.

Total length 50 cm, packed length 20 cm, packing 5 mm ODS Hypersil, voltage 15 kV, isocratic separation using: a 90:10 acetonitrile-water; b 60:40 acetonitrile-water; c a gradient elution using a gradient from 60 to 90% acetonitrile in water in 5 min. Peaks: acetone (1), phenol (2), benzene (3), toluene (4), naphthalene (5), acenaphthylene (6), fluorene (7), anthracene (8), 1,2benzanthracene (9) 36 F. Svec Fig. 25. Effect of percentage of acetonitrile (A) and methanol (B) on electroosmotic mobility in a packed column.

Effect of pH of the mobile phase on linear flow velocity (1) and electrical current (2) in the monolithic capillary column. (Reprinted with permission from [149]. Copyright 1998 American Chemical Society). d. 2 MPa, injection, 5 kV for 3 s Capillary Electrochromatography: a Rapidly Emerging Separation Method 39 erably exceed those of the original buffer solution (5 mmol/l). As a result of this increase in ionic strength, the conductivity of the mobile phase increases, and much higher currents are observed.

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