By Weiming Xia, Huaxi Xu
Within the look for an efficient remedy for Alzheimer's ailment, APP is a distinct version protein that illustrates the good selection of simple and complicated characterization strategies on hand.
Exploring quite a few organic concepts to explain the constitution and serve as of this transmembrane protein, this article offers each one approach with distinct, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.
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Additional info for Amyloid precursor protein : a practical approach
Aurora, OH). 2. Chemicals for crystallization may be obtained from Fluka (Buchs, Switzerland) or Sigma-Aldrich (Sydney, Australia). 3. Amicon protein microconcentrators may be obtained from Amicon, Inc. (Beverly, MA). 2 METHOD 1. 5. 2. The protein is concentrated to 10 mg/ml in Amicon concentrators. 3. 5 pH units). 4. Grease the rims of the wells with petroleum jelly. 5. Mix 2 µl of protein with 2 µl of reservoir solution on a cover slip and hang the cover slip over 1 ml of reservoir solution.
Chem. 270, 4205, 1995. 13. Murayama, Y. et al. Cell surface receptor function of amyloid precursor protein that activates Ser/Thr kinases. Gerontology 42, 2, 1996. 14. Zheng, H. et al. beta-Amyloid precursor protein-deficient mice show reactive gliosis and decreased locomotor activity. Cell 81, 525, 1995. 15. Hendriks, L. and Van Broeckhoven, C. A beta A4 amyloid precursor protein gene and Alzheimer’s disease. Eur. J. Biochem. 237, 6, 1996. 16. P. Cellular actions of beta-amyloid precursor protein and its soluble and fibrillogenic derivatives.
Expression of beta-amyloid precursor protein-CD3gamma chimeras to demonstrate the selective generation of amyloid beta(1-40) and amyloid beta(142) peptides within secretory and endocytic compartments. J. Biol. Chem. 274, 32295, 1999. 21. U. et al. The cytoplasmic domain of the LDL receptor-related protein regulates multiple steps in APP processing. EMBO J. 21, 5691, 2002. 22. H. Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons.
Amyloid precursor protein : a practical approach by Weiming Xia, Huaxi Xu